Make eye contact and hold it.
Before the interview is over, ask for the job. Twice. In your own words. And with sincerity. I'm always amazed when interview subjects don't ask for the job. And most don't.
“It's nice sometimes to open up the heart a little and let some hurt come in. It proves you're still alive.”
Have a substantial, but relatively concise answer to "So, tell me about yourself."